HisG Epitope Tag Antibody AP
Western blot analysis of HisG was performed by loading 20 µg of whole cell extracts of untransfected HEK-293 cell lysate (lane1) and HEK-293 transiently expressing HisG-RPS6 (lane2) using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), Xcell SureLock™ Electrophoresis system (Product # EI0002), Novex sharp Pre-stained Protein Standard (LC5800), and iBlot® Dry Blotting System (IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5 % skim milk for atleast 1 hour at room temperature. HisG was detected at ~35 kDa using HisG - AP Mouse Monoclonal Antibody (Product # R942-25) at a 1:1000 dilution in 2.5 % skim milk incubated for 1 hour on a rocking platform. Chemiluminescent detection was performed using AP Chemiluminescence Detection Kit (SLF1022).
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